Overview
SCIENTIFIC SCORE
Moderately Effective
Based on 18 Researches
8.4
USERS' SCORE
Good
Based on 151 Reviews
8.4
Supplement Facts
Serving Size: 1 Veg Capsule
Amount Per Serving
%DV
Nattokinase (2,000 FU) (enzyme from Natto Extract) (Non-GMO soy derived)
100 mg
**
Top Medical Research Studies
9
Nattokinase shows strong clot-busting activity
We explored the effectiveness of nattokinase, a natural enzyme known for its role in breaking down blood clots, by studying its production and activity in a lab setting. Nattokinase was extracted from Bacillus subtilis, and we introduced this gene into E. coli bacteria to produce the enzyme at high levels.
Through our process, we purified the active recombinant nattokinase and tested its ability to dissolve blood clots. Our results were promising; the enzyme exhibited a fibrinolytic activity of 58FU/mg, which is notably five times more effective than the currently used drug, orokinase.
We optimized the conditions for producing nattokinase, including factors like temperature and nutrient concentrations, resulting in an impressive yield of 1805.50 mg/L. This improved production method paves the way for scaling up nattokinase's use in treating cardiovascular diseases related to blood clots. The findings emphasize nattokinase's potential as an effective thrombolytic agent.
Through our process, we purified the active recombinant nattokinase and tested its ability to dissolve blood clots. Our results were promising; the enzyme exhibited a fibrinolytic activity of 58FU/mg, which is notably five times more effective than the currently used drug, orokinase.
We optimized the conditions for producing nattokinase, including factors like temperature and nutrient concentrations, resulting in an impressive yield of 1805.50 mg/L. This improved production method paves the way for scaling up nattokinase's use in treating cardiovascular diseases related to blood clots. The findings emphasize nattokinase's potential as an effective thrombolytic agent.
Read More
9
We explored the fascinating impact of nattokinase, a natural enzyme known for its blood-thinning properties, particularly its effect on blood clots. In our investigation, we examined how nattokinase interacts with thrombin, a key protein involved in the clotting process, and identified this enzyme as a significant substrate for nattokinase.
By employing a stepwise computational strategy, we modeled the interactions between nattokinase and thrombin to pinpoint specific cleavage sites. This innovative approach allowed us to validate our predictions through mass spectrometry and functional assays, solidifying our understanding of how nattokinase works at a molecular level.
Our findings reveal that nattokinase might play a vital role in breaking the cycle of inflammation and thrombosis by targeting thrombin, thus enhancing its therapeutic potential for cardiovascular health. Overall, this study sheds light on the crucial interactions between these proteins and underscores the importance of nattokinase in managing blood clot dynamics.
By employing a stepwise computational strategy, we modeled the interactions between nattokinase and thrombin to pinpoint specific cleavage sites. This innovative approach allowed us to validate our predictions through mass spectrometry and functional assays, solidifying our understanding of how nattokinase works at a molecular level.
Our findings reveal that nattokinase might play a vital role in breaking the cycle of inflammation and thrombosis by targeting thrombin, thus enhancing its therapeutic potential for cardiovascular health. Overall, this study sheds light on the crucial interactions between these proteins and underscores the importance of nattokinase in managing blood clot dynamics.
Read More
9
We aimed to explore how nattokinase, a key enzyme in natto—a traditional Japanese fermented soy product—affects blood clots. Our focus was on a newly isolated strain from Indonesian natto, named G8, which showed promising fibrinolytic activity, meaning its ability to dissolve blood clots.
Through various fibrinolytic assays, we discovered that both nattokinase and G8 could effectively dissolve clots from whole blood and euglobulin, indicating their potential in managing vascular obstructive diseases. A closer look at G8 revealed that it can directly break down fibrin, which is crucial in clot formation.
Interestingly, the crude extract of G8 demonstrated six distinct bands of activity on zymography, with the most potent activity found at a molecular weight of 20.0 kDa. This suggests that G8 contains a variety of fibrinolytic enzymes, potentially including nattokinase and others that support its clot-dissolving abilities. In summary, we observed that G8 exhibits strong fibrinolytic properties, supported by multiple enzymes working together to aid in clot dissolution.
Through various fibrinolytic assays, we discovered that both nattokinase and G8 could effectively dissolve clots from whole blood and euglobulin, indicating their potential in managing vascular obstructive diseases. A closer look at G8 revealed that it can directly break down fibrin, which is crucial in clot formation.
Interestingly, the crude extract of G8 demonstrated six distinct bands of activity on zymography, with the most potent activity found at a molecular weight of 20.0 kDa. This suggests that G8 contains a variety of fibrinolytic enzymes, potentially including nattokinase and others that support its clot-dissolving abilities. In summary, we observed that G8 exhibits strong fibrinolytic properties, supported by multiple enzymes working together to aid in clot dissolution.
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Most Useful Reviews
10
Eliminated headache
151 people found this helpful
I highly recommend it! The swelling has gone down, and my blood is flowing well. I began taking it during the pandemic and since then, I've not had a headache, which is remarkable for me.
Read More
9
Blood clot prevention
119 people found this helpful
Nattokinase is derived from fermented soybeans. It has a broad range of benefits, including heart health and reducing blood clots. I recommend it during the pandemic to reduce blood clot risks. This is not medical advice.
Read More
10
Supports circulatory system
113 people found this helpful
Nattokinase helps dilute blood and dissolve blood clots. It's an enzyme that promotes a healthy circulatory system and lowers the risk of coronary heart disease and stroke. The quality is excellent.
Read More
Medical Researches
SCIENTIFIC SCORE
Moderately Effective
Based on 18 Researches
8.4
- All Researches
9
We explored the impact of nattokinase, a natural enzyme known for breaking down blood clots, particularly in the context of acute myocardial infarction (MI), a serious heart condition often caused by these clots. The study investigated a special formulation combining nattokinase with a manganese oxide nanozyme, designed to enhance its effectiveness and stability.
Through tail vein injections into a mouse model, the combination of manganese oxide and nattokinase showed promising results. We observed that this innovative treatment not only helped dissolve clots but also countered oxidative stress—an issue that often exacerbates heart damage. The results indicated significant improvements in restoring blood flow and reducing heart injury.
Overall, our findings suggest that this nattokinase-loaded nanozyme could represent a valuable new tool in treating acute MI, potentially paving the way for better therapeutic strategies in the clinical setting.
Through tail vein injections into a mouse model, the combination of manganese oxide and nattokinase showed promising results. We observed that this innovative treatment not only helped dissolve clots but also countered oxidative stress—an issue that often exacerbates heart damage. The results indicated significant improvements in restoring blood flow and reducing heart injury.
Overall, our findings suggest that this nattokinase-loaded nanozyme could represent a valuable new tool in treating acute MI, potentially paving the way for better therapeutic strategies in the clinical setting.
Read More
9
We explored the effectiveness of a novel treatment combining nattokinase with magnetic vortex nanorobots for tackling blood clots. The study cleverly used these tiny robots, which are about 70 nanometers in size, powered by dual-frequency magnetic fields to improve drug delivery and enhance the effects of nattokinase.
By assembling into chain-like formations, these nanorobots can navigate through microvascular systems and directly target clots, creating mechanical forces to disrupt them efficiently. The combination of a low-frequency rotating field and a high-frequency alternating field helped not only to penetrate the thrombus but also to release nattokinase effectively.
Our findings revealed that this innovative approach, termed dual frequency-regulated magnetothrombolysis, achieved an impressive vasculature recanalization rate of 81.0% in thrombotic mice. This suggests that enhancing the therapeutic qualities of nattokinase with advanced nanotechnology represents a promising pathway for resolving blood clots more effectively.
By assembling into chain-like formations, these nanorobots can navigate through microvascular systems and directly target clots, creating mechanical forces to disrupt them efficiently. The combination of a low-frequency rotating field and a high-frequency alternating field helped not only to penetrate the thrombus but also to release nattokinase effectively.
Our findings revealed that this innovative approach, termed dual frequency-regulated magnetothrombolysis, achieved an impressive vasculature recanalization rate of 81.0% in thrombotic mice. This suggests that enhancing the therapeutic qualities of nattokinase with advanced nanotechnology represents a promising pathway for resolving blood clots more effectively.
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9
We explored how a combination of nattokinase (NK) and heparin (HP) can tackle cancer-related thrombosis (CAT), a common issue in cancer patients that can worsen their condition and quality of life. In our approach, we developed an electrostatic complex of these two agents to mitigate the toxicity typically associated with intravenous NK administration.
Our findings were quite promising. We observed that this NK-HP complex not only addressed the toxicity of NK but also demonstrated strong abilities to break down blood clots. The combination therapy was able to prevent new thrombosis from forming, significantly aiding its use in advanced tumors. Additionally, we found that administering NK-HP alongside specially formulated doxorubicin liposomes enhanced the treatment's effectiveness. This improvement was attributed to reduced fibrin levels in tumors and increased breakdown products, pointing to successful clot dissolution.
Importantly, our work highlights a potential breakthrough in managing CAT in cancer treatment, suggesting that our NK-HP method could be an effective strategy for improving outcomes in patients facing advanced tumors.
Our findings were quite promising. We observed that this NK-HP complex not only addressed the toxicity of NK but also demonstrated strong abilities to break down blood clots. The combination therapy was able to prevent new thrombosis from forming, significantly aiding its use in advanced tumors. Additionally, we found that administering NK-HP alongside specially formulated doxorubicin liposomes enhanced the treatment's effectiveness. This improvement was attributed to reduced fibrin levels in tumors and increased breakdown products, pointing to successful clot dissolution.
Importantly, our work highlights a potential breakthrough in managing CAT in cancer treatment, suggesting that our NK-HP method could be an effective strategy for improving outcomes in patients facing advanced tumors.
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9
We explored the effectiveness of nattokinase in helping dissolve blood clots while also addressing inflammation. The study successfully created amphiphilic block polymer-based nanovesicles that release both the anti-inflammatory drug indomethacin and nattokinase specifically at sites where reactive oxygen species (ROS) are plentiful—like inflamed or thrombotic tissues.
The combination of these drugs is significant; however, evaluating nattokinase's isolated effect is complex due to its combined use with indomethacin. The dual-action nanovesicles showed promising results in a mouse model of thrombosis, enhancing treatment outcomes by quickening the release of both drugs in response to inflammatory conditions.
Despite the potential of nattokinase, the findings indicate that its effect cannot be isolated from that of indomethacin, making it difficult to assess its individual contribution to thrombolytic success.
The combination of these drugs is significant; however, evaluating nattokinase's isolated effect is complex due to its combined use with indomethacin. The dual-action nanovesicles showed promising results in a mouse model of thrombosis, enhancing treatment outcomes by quickening the release of both drugs in response to inflammatory conditions.
Despite the potential of nattokinase, the findings indicate that its effect cannot be isolated from that of indomethacin, making it difficult to assess its individual contribution to thrombolytic success.
Read More
9
Nattokinase shows strong clot-busting activity
We explored the effectiveness of nattokinase, a natural enzyme known for its role in breaking down blood clots, by studying its production and activity in a lab setting. Nattokinase was extracted from Bacillus subtilis, and we introduced this gene into E. coli bacteria to produce the enzyme at high levels.
Through our process, we purified the active recombinant nattokinase and tested its ability to dissolve blood clots. Our results were promising; the enzyme exhibited a fibrinolytic activity of 58FU/mg, which is notably five times more effective than the currently used drug, orokinase.
We optimized the conditions for producing nattokinase, including factors like temperature and nutrient concentrations, resulting in an impressive yield of 1805.50 mg/L. This improved production method paves the way for scaling up nattokinase's use in treating cardiovascular diseases related to blood clots. The findings emphasize nattokinase's potential as an effective thrombolytic agent.
Through our process, we purified the active recombinant nattokinase and tested its ability to dissolve blood clots. Our results were promising; the enzyme exhibited a fibrinolytic activity of 58FU/mg, which is notably five times more effective than the currently used drug, orokinase.
We optimized the conditions for producing nattokinase, including factors like temperature and nutrient concentrations, resulting in an impressive yield of 1805.50 mg/L. This improved production method paves the way for scaling up nattokinase's use in treating cardiovascular diseases related to blood clots. The findings emphasize nattokinase's potential as an effective thrombolytic agent.
Read More
User Reviews
USERS' SCORE
Good
Based on 151 Reviews
8.4
- All Reviews
- Positive Reviews
- Negative Reviews
10
Eliminated headache
151 people found this helpful
I highly recommend it! The swelling has gone down, and my blood is flowing well. I began taking it during the pandemic and since then, I've not had a headache, which is remarkable for me.
Read More
9
Blood clot prevention
119 people found this helpful
Nattokinase is derived from fermented soybeans. It has a broad range of benefits, including heart health and reducing blood clots. I recommend it during the pandemic to reduce blood clot risks. This is not medical advice.
Read More
10
Supports circulatory system
113 people found this helpful
Nattokinase helps dilute blood and dissolve blood clots. It's an enzyme that promotes a healthy circulatory system and lowers the risk of coronary heart disease and stroke. The quality is excellent.
Read More
9
Prevents clots
36 people found this helpful
Nattokinase effectively thins blood and dissolves blood clots. It supports circulation, reduces cholesterol levels, and strengthens bones. Importantly, it shouldn't be combined with other blood-thinning medications.
Read More
10
Improves circulation
30 people found this helpful
Nattokinase, a soy-derived enzyme, dissolves fibrin in blood vessels and lowers cholesterol levels, thus promoting heart health. I have been taking it for four months without any issues.
Read More
Frequently Asked Questions
10
Improves circulation
30 people found this helpful
Nattokinase, a soy-derived enzyme, dissolves fibrin in blood vessels and lowers cholesterol levels, thus promoting heart health. I have been taking it for four months without any issues.
10
Restored normal blood
4 people found this helpful
It worked great for me! My platelet levels were high, and after taking it twice daily for three months, everything returned to normal. I’m very satisfied with this product.
10
Eliminated headache
151 people found this helpful
I highly recommend it! The swelling has gone down, and my blood is flowing well. I began taking it during the pandemic and since then, I've not had a headache, which is remarkable for me.
9
Reduced symptoms and conditions
15 people found this helpful
I highly recommend Nattokinase! The quality is excellent, and it's helped me tremendously after COVID. It thins the blood, reduces fibrin, and normalises blood pressure, among other benefits. I've noticed reduced swelling in my legs and improved overall well-being. I take 1 capsule twice a day.
9
Lowered blood pressure
5 people found this helpful
I have heard much about nattokinase and decided to try it myself. My borderline blood pressure has improved from 130/85 to 114/72 since I started taking this supplement. I look forward to seeing my blood work results!
9
Relieves discomfort
8 people found this helpful
This excellent product has significantly helped my varicose veins, which worsened a year ago. I sought a natural remedy, and Nattokinase has relieved much discomfort by assisting with blood thinning. When I ran out, I noticed a considerable difference, so I stocked up to ensure I don't go without it again, especially to prevent blood clots.
9
Prevents blood clots
7 people found this helpful
Nattokinase has been instrumental in protecting against deep vein thrombosis (DVT), minimising the stickiness of my platelets. I wish to avoid another blood clot experience and prefer non-medical solutions. It pairs well with L-Citrulline, anchoring my strategy against risks associated with DVT.
9
Prevents clots
36 people found this helpful
Nattokinase effectively thins blood and dissolves blood clots. It supports circulation, reduces cholesterol levels, and strengthens bones. Importantly, it shouldn't be combined with other blood-thinning medications.
9
Effective alternative
6 people found this helpful
A wonderful blood thinner! After illness with COVID, the doctors prescribed a powerful blood thinner for a month. We found nattokinase to be an ideal, safer replacement, and it works well for long-term use.
8
Nattokinase: Effective blood-clot treatment
We aimed to understand how nattokinase, an enzyme derived from fermented soya beans, affects blood clots. By screening samples for bacteria that produce fibrinolytic proteases, we discovered a promising strain named MRS18.
This strain was particularly effective, showing remarkable clot-lysing abilities in our tests. When we evaluated its performance, MRS18 had a clot lysis percentage of 61.7% after 120 minutes, which increased to a striking 656% after further purification of the enzyme.
Our findings indicate that nattokinase from MRS18 has substantial potential in breaking down blood clots. Given its effectiveness and relative cost-effectiveness compared to other treatments, this enzyme could play a significant role in medical treatments related to thrombosis.
This strain was particularly effective, showing remarkable clot-lysing abilities in our tests. When we evaluated its performance, MRS18 had a clot lysis percentage of 61.7% after 120 minutes, which increased to a striking 656% after further purification of the enzyme.
Our findings indicate that nattokinase from MRS18 has substantial potential in breaking down blood clots. Given its effectiveness and relative cost-effectiveness compared to other treatments, this enzyme could play a significant role in medical treatments related to thrombosis.
9
We explored the impact of nattokinase, a natural enzyme known for breaking down blood clots, particularly in the context of acute myocardial infarction (MI), a serious heart condition often caused by these clots. The study investigated a special formulation combining nattokinase with a manganese oxide nanozyme, designed to enhance its effectiveness and stability.
Through tail vein injections into a mouse model, the combination of manganese oxide and nattokinase showed promising results. We observed that this innovative treatment not only helped dissolve clots but also countered oxidative stress—an issue that often exacerbates heart damage. The results indicated significant improvements in restoring blood flow and reducing heart injury.
Overall, our findings suggest that this nattokinase-loaded nanozyme could represent a valuable new tool in treating acute MI, potentially paving the way for better therapeutic strategies in the clinical setting.
Through tail vein injections into a mouse model, the combination of manganese oxide and nattokinase showed promising results. We observed that this innovative treatment not only helped dissolve clots but also countered oxidative stress—an issue that often exacerbates heart damage. The results indicated significant improvements in restoring blood flow and reducing heart injury.
Overall, our findings suggest that this nattokinase-loaded nanozyme could represent a valuable new tool in treating acute MI, potentially paving the way for better therapeutic strategies in the clinical setting.
9
We explored the potential of nattokinase (NK) as a treatment for blood clots, known for its strong thrombolytic properties. The focus was on creating NK-conjugated magnetite nanoparticles (NK-MNPs) to effectively deliver NK directly to the site of thrombus, enhancing its efficacy.
During our research, we prepared magnetite nanoparticles using a combination of Fe₃ O₄, carboxymethyl chitosan, and sodium alginate. These nanoparticles encapsulated NK, creating a delivery system with a mean diameter of 168.9 nm and a negative zeta potential of -33.8 mV. When tested, we found that around 65.24% of NK was released within 12 hours, demonstrating a sustained release mechanism.
The results of our thrombolysis experiments underscored the effectiveness of this system. NK-MNPs showed a high degree of light transmittance at 90%, indicating successful targeting. Additionally, we noted that the hemolysis rate remained below 5% at effective enzyme activity levels. Importantly, after 90 days of storage at 4 °C, NK-MNPs retained over 90% of their enzyme activity, suggesting good stability.
In conclusion, our findings point to NK-MNPs being a promising option for improving thrombolytic treatment, combining both sustained release and targeted delivery to enhance the treatment of thrombolic diseases.
During our research, we prepared magnetite nanoparticles using a combination of Fe₃ O₄, carboxymethyl chitosan, and sodium alginate. These nanoparticles encapsulated NK, creating a delivery system with a mean diameter of 168.9 nm and a negative zeta potential of -33.8 mV. When tested, we found that around 65.24% of NK was released within 12 hours, demonstrating a sustained release mechanism.
The results of our thrombolysis experiments underscored the effectiveness of this system. NK-MNPs showed a high degree of light transmittance at 90%, indicating successful targeting. Additionally, we noted that the hemolysis rate remained below 5% at effective enzyme activity levels. Importantly, after 90 days of storage at 4 °C, NK-MNPs retained over 90% of their enzyme activity, suggesting good stability.
In conclusion, our findings point to NK-MNPs being a promising option for improving thrombolytic treatment, combining both sustained release and targeted delivery to enhance the treatment of thrombolic diseases.
8
We evaluated the potential of nattokinase, a natural enzyme known for breaking down blood clots, in treating patients with vascular diseases such as deep vein thrombosis and venous insufficiency. Our study involved 153 patients who were given a daily dose of 100 mg of nattokinase, either alone or alongside standard anticoagulants.
The results showed that nattokinase improved clinical symptoms significantly without causing any adverse reactions or drug interactions. We observed that while other treatments were included, nattokinase still played a crucial role in enhancing the patients' conditions. Moreover, throughout the follow-up period, there were no new cases of vascular diseases reported.
This study underscores the importance of monitoring patient progress and adjusting treatments as necessary while administering fibrinolytic medications. Ultimately, we can conclude that nattokinase offers a promising and safe option for both preventing and treating vascular diseases.
The results showed that nattokinase improved clinical symptoms significantly without causing any adverse reactions or drug interactions. We observed that while other treatments were included, nattokinase still played a crucial role in enhancing the patients' conditions. Moreover, throughout the follow-up period, there were no new cases of vascular diseases reported.
This study underscores the importance of monitoring patient progress and adjusting treatments as necessary while administering fibrinolytic medications. Ultimately, we can conclude that nattokinase offers a promising and safe option for both preventing and treating vascular diseases.
8
We explored how nattokinase, a beneficial enzyme derived from the traditional Japanese food natto, can help in preventing and treating blood clots. However, we know that when taken orally, nattokinase often loses its effectiveness due to the harsh environment of the stomach, especially the acidic gastric juices.
To tackle this challenge, we developed a unique delivery system using chitosan microparticles as a core to encapsulate nattokinase and protect it. This core is then shielded by a casein-based layer to further enhance its protective capabilities, thanks to a special crosslinking process. Through rigorous testing, both in the lab and with living subjects, we found that this double-layer structure successfully safeguards nattokinase from destruction in the stomach and ensures its gradual release in the intestines.
Our findings indicate that this innovative delivery system can preserve the enzyme's activity and improve its absorption, making it a promising candidate for use as a functional food ingredient aimed at fighting thrombosis. This suggests that incorporating such a system might provide real benefits in cardiovascular health.
To tackle this challenge, we developed a unique delivery system using chitosan microparticles as a core to encapsulate nattokinase and protect it. This core is then shielded by a casein-based layer to further enhance its protective capabilities, thanks to a special crosslinking process. Through rigorous testing, both in the lab and with living subjects, we found that this double-layer structure successfully safeguards nattokinase from destruction in the stomach and ensures its gradual release in the intestines.
Our findings indicate that this innovative delivery system can preserve the enzyme's activity and improve its absorption, making it a promising candidate for use as a functional food ingredient aimed at fighting thrombosis. This suggests that incorporating such a system might provide real benefits in cardiovascular health.
References
- Zhang Z, Gao F, Mao J, Liu J, Zeng Z, et al. Biophysiochemically favorable, antithrombotic and pro-endothelial coordination compound nanocoating of copper (II) with protocatechuic acid & nattokinase on flow-diverting stents. Colloids Surf B Biointerfaces. 2025;249:114509. doi:10.1016/j.colsurfb.2025.114509
- Shi Y, Liu C, Gui Y, Guo Y, Zhang Y, et al. A Nattokinase-Loaded Nanozyme for Alleviating Acute Myocardial Infarction via Thrombolysis and Antioxidation. Adv Healthc Mater. 2024. doi:10.1002/adhm.202402763
- Gao R, Zhang W, Chen X, Shen J, Qin Y, et al. Dual Frequency-Regulated Magnetic Vortex Nanorobots Empower Nattokinase for Focalized Microvascular Thrombolysis. ACS Nano. 2024;18:29492. doi:10.1021/acsnano.4c04331
- Wang D, Kou Y, Guo T, Duan L, Chen J, et al. Intravenous injection of nattokinase-heparin electrostatic complex improves the therapeutic effect of advanced tumors by dissolving cancer-related thrombosis. Life Sci. 2024;355:122935. doi:10.1016/j.lfs.2024.122935
- Coutinho SB, Shirodkar RR, Kanjirakkandy A, Arulmozhi V, Vaithilingam M, et al. Production of Nattokinase from MRS18: A Bacterial Strain Isolated from Fermented Beans. Cardiovasc Hematol Agents Med Chem. 2024;22:223. doi:10.2174/0118715257275639231219054353
- Feng H, Liu C, Liu Q, Wang J, Zeng Y, et al. Study on the transport and internalisation mechanism of dietary supplement nattokinase in the small intestine using animal and Caco-2 cell monolayer models. Xenobiotica. 2023;53:670. doi:10.1080/00498254.2023.2284249
- Xia Y, Chen R, Ke Y, Han Q, Ma Z, et al. ROS-responsive phenylboronic ester-based nanovesicles as multifunctional drug delivery systems for the treatment of inflammatory and thrombotic complications. Biomater Sci. 2023;11:7805. doi:10.1039/d3bm01427d
- Li Y, Chen L, Tang X, Zhu W, Ma A, et al. Combined Computer-Aided Predictors to Improve the Thermostability of Nattokinase. Foods. 2023;12. doi:10.3390/foods12163045
- Modi A, Raval I, Doshi P, Joshi M, Joshi C, et al. Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM. Protein Expr Purif. 2023;203:106198. doi:10.1016/j.pep.2022.106198
- Wang Y, Wang H, Zhang Y, Xu F, Wang J, et al. Stepwise Strategy to Identify Thrombin as a Hydrolytic Substrate for Nattokinase. J Chem Inf Model. 2022;62:5780. doi:10.1021/acs.jcim.2c00978
- Pinontoan R, Sanjaya A, Jo J. Fibrinolytic characteristics of G8 isolated from natto. Biosci Microbiota Food Health. 2021;40:144. doi:10.12938/bmfh.2020-071
- Zhang X, Tong Y, Wang J, Lyu X, Yang R. Screening of a Bacillus subtilis strain producing both nattokinase and milk-clotting enzyme and its application in fermented milk with thrombolytic activity. J Dairy Sci. 2021;104:9437. doi:10.3168/jds.2020-19756
- Gallelli G, Di Mizio G, Palleria C, Siniscalchi A, Rubino P, et al. Data Recorded in Real Life Support the Safety of Nattokinase in Patients with Vascular Diseases. Nutrients. 2021;13. doi:10.3390/nu13062031
- Liu S, Zhu J, Liu C, Li J, Li Z, et al. Synthesis of sustained release/controlled release nanoparticles carrying nattokinase and their application in thrombolysis. Pharmazie. 2021;76:145. doi:10.1691/ph.2021.0155
- Li X, Yang X, Umar M, Zhang Z, Luo W, et al. Expression of a novel dual-functional polypeptide and its pharmacological action research. Life Sci. 2021;267:118890. doi:10.1016/j.lfs.2020.118890
- Zhang X, Lyu X, Tong Y, Wang J, Ye J, et al. Chitosan/casein based microparticles with a bilayer shell-core structure for oral delivery of nattokinase. Food Funct. 2020;11:10799. doi:10.1039/d0fo02349c
- Liu M, Xu Z, Wang Z, Wang D, Yang M, et al. Lipid-lowering, antihypertensive, and antithrombotic effects of nattokinase combined with red yeast rice in patients with stable coronary artery disease: a randomized, double-blinded, placebo-controlled trial. Front Nutr. 2024;11:1380727. doi:10.3389/fnut.2024.1380727
- Takagaki S, Suzuki M, Suzuki E, Hasumi K. Unsaturated fatty acids enhance the fibrinolytic activity of subtilisin NAT (nattokinase). J Food Biochem. 2020;44:e13326. doi:10.1111/jfbc.13326
